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Pregled bibliografske jedinice broj: 191845

Arginine kinase in the demosponge Suberites domuncula: regulation of its expression and catalytic activity by silicic acid


Perovic-Ottstadt, S.; Wiens, M.; Schroder, H.C.; Batel, Renato; Giovine, M.; Krasko, A.; Muller, I.M.; Muller, W.E.G.
Arginine kinase in the demosponge Suberites domuncula: regulation of its expression and catalytic activity by silicic acid // Journal of experimental biology, 208 (2005), 4; 637-646 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 191845 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Arginine kinase in the demosponge Suberites domuncula: regulation of its expression and catalytic activity by silicic acid

Autori
Perovic-Ottstadt, S. ; Wiens, M. ; Schroder, H.C. ; Batel, Renato ; Giovine, M. ; Krasko, A. ; Muller, I.M. ; Muller, W.E.G.

Izvornik
Journal of experimental biology (0022-0949) 208 (2005), 4; 637-646

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Sponge. Arginine kinase. Siliceous spicule. Suberites domuncula. Primmorph. Energy metabolism.

Sažetak
In Demospongiae (phylum Porifera) the formation of the siticeous skeleton, composed of spicules, is an energetically expensive reaction. The present study demonstrates that primmorphs from the demosponge Suberites domuncula express the gene for arginine kinase after exposure to exogenous silicic acid. The deduced sponge arginine kinase sequence displays the two characteristic domains of the ATP:guanido phosphotransferases ; it can be grouped to the 'usual' mono-domain 40kDa guanidino kinases (arginine kinases). Phylogenetic studies indicate that the metazoan guanidino kinases evolved from this ancestral sponge enzyme ; among them are also the 'unusual' two-domain 80 kDa guanidino kinases. The high expression level of the arginine kinase gene was already measurable 1 day after Summary addition of silicic acid by northern blot, as well as by in situ hybridization analysis. Parallel determinations of enzyme activity confirmed that high levels of arginine kinase are present in primmorphs that had been exposed for 1-5 days to silicic acid. Finally, transmission electron-microscopical studies showed that primmorphs containing high levels of arginine kinase also produce siliceous spicules. These data highlight that silicic acid is an inorganic morphogenetic factor that induces the expression of the arginine kinase, which in turn probably catalyzes the reversible transfer of high-energy phosphoryl groups.

Izvorni jezik
Engleski

Znanstvena područja
Sociologija



POVEZANOST RADA


Projekti:
0098114

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Renato Batel (autor)

Citiraj ovu publikaciju:

Perovic-Ottstadt, S.; Wiens, M.; Schroder, H.C.; Batel, Renato; Giovine, M.; Krasko, A.; Muller, I.M.; Muller, W.E.G.
Arginine kinase in the demosponge Suberites domuncula: regulation of its expression and catalytic activity by silicic acid // Journal of experimental biology, 208 (2005), 4; 637-646 (međunarodna recenzija, članak, znanstveni)
Perovic-Ottstadt, S., Wiens, M., Schroder, H., Batel, R., Giovine, M., Krasko, A., Muller, I. & Muller, W. (2005) Arginine kinase in the demosponge Suberites domuncula: regulation of its expression and catalytic activity by silicic acid. Journal of experimental biology, 208 (4), 637-646.
@article{article, year = {2005}, pages = {637-646}, keywords = {Sponge. Arginine kinase. Siliceous spicule. Suberites domuncula. Primmorph. Energy metabolism.}, journal = {Journal of experimental biology}, volume = {208}, number = {4}, issn = {0022-0949}, title = {Arginine kinase in the demosponge Suberites domuncula: regulation of its expression and catalytic activity by silicic acid}, keyword = {Sponge. Arginine kinase. Siliceous spicule. Suberites domuncula. Primmorph. Energy metabolism.} }
@article{article, year = {2005}, pages = {637-646}, keywords = {Sponge. Arginine kinase. Siliceous spicule. Suberites domuncula. Primmorph. Energy metabolism.}, journal = {Journal of experimental biology}, volume = {208}, number = {4}, issn = {0022-0949}, title = {Arginine kinase in the demosponge Suberites domuncula: regulation of its expression and catalytic activity by silicic acid}, keyword = {Sponge. Arginine kinase. Siliceous spicule. Suberites domuncula. Primmorph. Energy metabolism.} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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