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Pregled bibliografske jedinice broj: 191841

Silica transport in the demosponge Suberites domuncula: fluorescence emission analysis using the PDMPO probe and cloning of a potential transporter


Schroder, H.C.; Perovic-Ottstadt, S.; Rothenberger, M.; Wiens, M.; Schwertner, H.; Batel, Renato; Korzhev, M.; Muller, I.M.; Muller, W.E.G.
Silica transport in the demosponge Suberites domuncula: fluorescence emission analysis using the PDMPO probe and cloning of a potential transporter // Biochemical journal (London. 1984), 381 (2004), 665-673 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 191841 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Silica transport in the demosponge Suberites domuncula: fluorescence emission analysis using the PDMPO probe and cloning of a potential transporter

Autori
Schroder, H.C. ; Perovic-Ottstadt, S. ; Rothenberger, M. ; Wiens, M. ; Schwertner, H. ; Batel, Renato ; Korzhev, M. ; Muller, I.M. ; Muller, W.E.G.

Izvornik
Biochemical journal (London. 1984) (0264-6021) 381 (2004); 665-673

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Na+/hco3-[si(oh)(4)] (nbcsa)-related transporter. 2-(4-pyridyl)-5-{;;; [4-(2-dimethylaminoethylaminocarbamoyl)methoxy]phenyl};;; oxazole. (pdmpo). Silica uptake. Spicule formation. Sponges. Suberites domuncula.

Sažetak
Silicon is, besides oxygen, the most abundant element on earth. Only two taxa use this element as a major constituent of their skeleton, namely sponges (phylum Porifera) and unicellular diatoms. Results from combined cytobiological and molecular-biological techniques suggest that, in the demosponge Suberites domuncula, silicic acid is taken up by a transporter. Incubation of cells with the fluorescent silica tracer PDMPO [2-(4-pyridyl)-5-{; ; ; [4-(2-dimethylaminoethylaminocarbamoyl)methoxy]phenyl}; ; ; -oxazole] showed a response to silicic acid by an increase in fluorescence ; this process is temperature-dependent and can be blocked by DIDS (4, 4-di-isothiocyanatostilbene-2, 2-disulphonic acid). The putative NBC (Na+/HCO3-) transporter was identified, cloned and analysed. The deduced protein comprises all signatures characteristic of those molecules, and phylogenetic analysis also classifies it to the NBC transporter family. This cDNA was used to demonstrate that the expression of the gene is strongly up-regulated after treatment of cells with silicic acid. In situ hybridization demonstrated that the expression of the sponge transporter occurs in those cells that are located adjacent to the spicules (the skeletal element of the animal) or in areas in which spicule formation occurs. We conclude that this transporter is involved in silica uptake and have therefore termed it the NBCSA {; ; ; Na+/HCO3-[Si(OH)(4)]}; ; ; co-transporter

Izvorni jezik
Engleski

Znanstvena područja
Računarstvo



POVEZANOST RADA


Projekti:
0098114

Ustanove:
Fakultet elektrotehnike i računarstva, Zagreb

Profili:

Avatar Url Renato Batel (autor)


Citiraj ovu publikaciju:

Schroder, H.C.; Perovic-Ottstadt, S.; Rothenberger, M.; Wiens, M.; Schwertner, H.; Batel, Renato; Korzhev, M.; Muller, I.M.; Muller, W.E.G.
Silica transport in the demosponge Suberites domuncula: fluorescence emission analysis using the PDMPO probe and cloning of a potential transporter // Biochemical journal (London. 1984), 381 (2004), 665-673 (međunarodna recenzija, članak, znanstveni)
Schroder, H., Perovic-Ottstadt, S., Rothenberger, M., Wiens, M., Schwertner, H., Batel, R., Korzhev, M., Muller, I. & Muller, W. (2004) Silica transport in the demosponge Suberites domuncula: fluorescence emission analysis using the PDMPO probe and cloning of a potential transporter. Biochemical journal (London. 1984), 381, 665-673.
@article{article, year = {2004}, pages = {665-673}, keywords = {Na+/hco3-[si(oh)(4)] (nbcsa)-related transporter. 2-(4-pyridyl)-5-{, , , [4-(2-dimethylaminoethylaminocarbamoyl)methoxy]phenyl}, , , oxazole. (pdmpo). Silica uptake. Spicule formation. Sponges. Suberites domuncula.}, journal = {Biochemical journal (London. 1984)}, volume = {381}, issn = {0264-6021}, title = {Silica transport in the demosponge Suberites domuncula: fluorescence emission analysis using the PDMPO probe and cloning of a potential transporter}, keyword = {Na+/hco3-[si(oh)(4)] (nbcsa)-related transporter. 2-(4-pyridyl)-5-{, , , [4-(2-dimethylaminoethylaminocarbamoyl)methoxy]phenyl}, , , oxazole. (pdmpo). Silica uptake. Spicule formation. Sponges. Suberites domuncula.} }
@article{article, year = {2004}, pages = {665-673}, keywords = {Na+/hco3-[si(oh)(4)] (nbcsa)-related transporter. 2-(4-pyridyl)-5-{, , , [4-(2-dimethylaminoethylaminocarbamoyl)methoxy]phenyl}, , , oxazole. (pdmpo). Silica uptake. Spicule formation. Sponges. Suberites domuncula.}, journal = {Biochemical journal (London. 1984)}, volume = {381}, issn = {0264-6021}, title = {Silica transport in the demosponge Suberites domuncula: fluorescence emission analysis using the PDMPO probe and cloning of a potential transporter}, keyword = {Na+/hco3-[si(oh)(4)] (nbcsa)-related transporter. 2-(4-pyridyl)-5-{, , , [4-(2-dimethylaminoethylaminocarbamoyl)methoxy]phenyl}, , , oxazole. (pdmpo). Silica uptake. Spicule formation. Sponges. Suberites domuncula.} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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