engleski

Non-invasive and continuous recording of auxin fluxes in intact root apex with a carbon-nanotube-modified and self-referencing microelectrode

Auxin (also known as indole-3-acetic acid, IAA) represents an ancient signalling molecule of plants which also exerts bioactive actions on yeast and animal cells. Importantly, IAA emerges as a new anti-cancer agent due to the ability of oxidatively activated IAA to kill selectively tumor cells. IAA acts as a pheromone-like molecule in brown algae while the hormone concept of IAA dominates current plant biology. However, recent advances also favour a morphogen-like and transmitter-like nature of IAA in plants, making this small molecule one of the most unique molecules in the eukaryotic superkingdom. Here we introduce new technology for continuous measurement of IAA fluxes in living cells, tissues, and whole organs which is based on a carbon-nanotube-modified and self-referencing microelectrode specific for IAA. This technique will not only advance our knowledge how IAA regulates plant development but it will also be applicable in medicine for its potential use in cancer therapy.

Auxin; carbon nanotube; IAA microsensor; maize root apex; transition zone

nije evidentirano