engleski

Paraoxonase phenotype distribution in a healthy population group from the Slavonski Brod Area

Paraoxonase (EC 3.1.8.1) is an esterase that hydrolyzes organophosphate compounds. The enzyme is associated with HDL and could protect LDL against peroxidation, which suggests a possible involvement of paraoxonase in the antiatherogenic properties of HDL. Several studies have shown that paraoxonase levels in humans have a distribution characteristic of two alleles, one with low (AA) and the other with high activity (BB). Paraoxonase also has arylesterase activity. We investigated paraoxonase and arylesterase activity of the enzyme as well as phenotype distribution of paraoxonase in sera of volunteer blood donors from the area of Slavonski Brod, Croatia. The distribution of paraoxonase activities in the absence of NaCl was polymodal and the activities ranged between 55 and 611 U/L while NaCl-stimulated activities ranged between 93 and 1437 U/L. The arylesterase activities ranged between 28 and 215 kU/L and the distribution was unimodal. Using the double substrate method to assign phenotypes on 145 healthy individuals selected among the blood donor volunteers, 39% individuals classified as AA homozygous low paraoxonase activity group, 48% individuals classified as AB heterozygous intermediate activity group and 13% individuals belong to BB homozygous high activity group. Conclusion. Distribution of the paraoxonase phenotypes was similar to that of other European populations.

Arylesterase; Lipoproteins; HDL; Paraoxonase; Phenotype

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