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Composition of volatiles from needles of Pinus nigra Arnold, P. sylvestris L. and their hybrids

The composition of needle volatiles of Pinus nigra Arnold (= ni), P. sylvestris L. (= sy) and their F1 and F2 hybrids (= F1 nisy, F2 nisy) was investigated with gas chromatography (GC) and gas chromatography / mass spectrometry (GC/MS). The number of detected components varied with species and hybrids (ni 141, sy 107, F1 nisy 129, F2 nisy 83). A total of 55 components was identified, of which 51 were unambigously identified and 4 were partially characterized. The identified components accounted for 94.4 % w/w sample of P. nigra, 90.1 % w/w sample of P. sylvestris, 91.4 % w/w sample of F1 hybrids, and 93.9 % w/w sample of F2 hybrids. All identified components were terpenes except trans-2-hexenal which is a n-alkene. The amount of monoterpenes was the highest (ni 65 %, sy 54 %, F1 nisy 64 %, F2 nisy 71 %), followed by sesquiterpenes (ni 27 %, sy 36 %, F1 nisy 27 %, F2 nisy 23 %) and diterpenes (ni 2.34 %, sy 0.2 %, F1 nisy 0.11 %, F2 nisy 0.16 %). Cluster analysis shows that the hybrids F1 and F2 are the most similar, and that both hybrid groups are more similar to European black pine (P. nigra) than to Scots pine (P. sylvestris).

Pinus nigra Arnold, P. sylvestris L., interspecific hybrids, volatiles, GC, GC/MS, terpenes, cluster analysis

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