engleski

Antisperm antibodies in semen, sera and follicular fluid of infertile patients ; relation to reproductive outcome after in vitro fertilization

PROBLEM: Data given in literature, regarding the influence of antisperm antibodies (ASA) in semen and/or sera on in vitro fertilization (IVF) procedure outcome are controversial. The aim of this study was to compare the prevalence of ASA in semen, peripheral blood and follicular fluid as well as to determine total immunoglobulin concentration in the serum and follicular fluid. Selected parameters were analysed with regards to IVF outcome. METHOD OF STUDY: The study enrolled 52 married couples. ASA in the semen was determined by direct immuno bead mixed antiglobulin reaction (MAR Screen test), while in the peripheral blood and follicular fluid was determined by indirect immuno bead MAR Screen test. Immunoglobulin (IgG, IgM and IgA) concentration in the follicular fluid and serum was determined by a liquid-phase immunoprecipitation assay with nephelometric end-point detection and analysed with regards to IVF outcome. RESULTS: Semen MAR test IgG was < 20% in 38 couples, and > 20% in 14 couples. Fertilization (73.2% vs. 71.5%) and pregnancy rates (28.9% vs. 28.57%) in both groups of patients were not significantly different. The results of direct and indirect MAR test were not associated with fertilization and pregnancy rates. Total serum IgG, IgM and IgA in infertile women were within normal ranges. Follicular fluid IgG was within normal values for serum samples, while IgA and IgM were decreased. CONCLUSION: The presence of ASA on sperm or in the serum and follicular fluid was not associated with in vitro fertilization outcome in the couples with good quality semen characteristic.

MAR; imunoglobulini; sjeme; serum; folikularna tekućina; IVF/ET

nije evidentirano