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Effect of splenectomy and low dose cyclophosphamide od susceptibility to active induction and reinduction of relapsing experimental allergic encephalomyelitis (R-EAE) in rats

Juvenile DA and F1 hybrid rats (AOxDA) were immunized with bovine brain white matter in complete Freund's adjuvant (CFA) to produce the ralapsing form of experimental allergic encephalomyelitis (R-EAE). Recovery from actively induced disease resulted in a different rate of susceptibility to active reinduction of disease in these high susceptible rats. Hybrids F1 (AOxDA) developed relative resistance, while DA rats where completely resistant to active reinduction of disease. The treatment with cyclophosphamide (20 mg/kg), administered two days before reimmunization, inhibited the resistance to EAE reinduction observed in DA rats, and increased the susceptibility to EAE reinduction in F1 hybrids. Rats which were splenectomized 12 days before the initial immunization were highly susceptible to active EAE reinduction. Howewer, when splenectomy was performed 4 days after encephalitogenic challenge (i.e. during the induction phase of disease), significantly lower susceptibility to active EAE reinduction was observed. These results suggest that low dose cyclophosphamide sensitive (suppressor) cells and spleen dependent suppressor activity could directly mediate the mechanisms of acquiring resistance to active disease reinduction.

splenectomy; cyclophosphamide; experimental allergic encephalomyelitis; rats

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