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Observation of cataractous changes in human eye lens by means of electron microscopy and Raman spectroscopy

The water insoluble fraction of proteins extracted from cataractous human lenses increases with the age [1] and the number of proteins involved precludes their isolation and characterization. Fluorescence recorded for tyrozine- and triptophane- containing crystallin proteins does not offer much discriminating data [2]. Raman spectroscopy was used to identify the chan-ges in the rat lenses during the artificially caused diabetes mellitus [3]. The 514.5 nm green line of argon laser was used as the excitation source (see Figure 1, bottom). Several human lenses distorted because of age related cataract were used as specimen for Fourier-transform Raman spectroscopy (Figure 2.) There is an overall agreement in positions of many bands (Table 1).

microscopy ; Raman ; cataract ; eye-lens

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