engleski

Seasonal atrazine contamination of drinking water in pig-breeding farms surroundings in agricultural and industrial areas of Croatia

Atrazine (2-chloro-4-ethylamino-6-isopropylamino- s-triazine) a s-triazine herbicide, has been widely used in Croatian agriculture. Due to atrazine extensive use and its biodegradation in nature within at least one year, atrazine residues are found in ground, surface, drain and drinking water. Graundwater downgradient from atrazine treated filds may show seasonal concentration peaks which could exceed the safe level. Therefore, the use of atrazine includes permanent control of its residues in water, particularly in relation to its use as a herbicidal chemical and groundwater contamination. Futhermore, the presence of atrazine in the environment and its possible ingestion via the water, food and feed chain, may present the risk for the animal and human health. The analysis of atrazine residues in soil can be performed by either colorimetry or high performance liquid chromatography (HPLC), and in water, soil and food by immunoassay in comparison with HPLC or gas chromatography/mass spectrometry (GS/MS). We describe the use of enzyme-linked immunosorbent assay (ELISA) for one- year seasonal monitoring of atrazine residues in drinking water from two differently situated pig- breeding farms in Croatia. Results obtained by ELISA were compared to those produced by HPLC.

atrazine ; water ; pig-breeding farm surroundings

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