Macromolecular prodrugs : VI. kinetic study of poly[alpha, beta-(N-2-hydroxyethyl-DL-aspartamide)]-ketoprofen hydrolysis (CROSBI ID 77557)
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Podaci o odgovornosti
Jakšić, Planinka ; Mlinarić-Majerski, Kata ; Zorc, Branka ; Dumić, Miljenko
engleski
Macromolecular prodrugs : VI. kinetic study of poly[alpha, beta-(N-2-hydroxyethyl-DL-aspartamide)]-ketoprofen hydrolysis
The kinetics of hydrolysis of ketoprofen from poly[alpha, beta-(N-2-hydroxyethyl-DL-aspartamide)]-ketoprofen conjugate (PHEA-Ket) in aqueous buffer solutions in the pH range 1.0-10.6 at 37 degrees C was studied. The hydrolysis of PHEA-Ket conjugate followed the pseudofirst-order kinetic. The following rate constants were obtained: k = 1.542 x 10(-2)h (-1) at pH = 1.0, k = 6.260 x 10(-3) h(-1) at pH = 9.2, and k = 0.155 h(-1) at pH = 10.6. At pH = 5.0 and 7.0 no significant hydrolysis was observed. The PHEA-Ket was also stable after exposure to several enzymatic systems in neutral medium. Determination of ketoprofen was performed by high performance liquid chromatography using diclofenac as the internal standard.
polymeric prodrug; ketoprofen; poly[alpha; beta-(n-2-hydroxyethyl-dl-aspartamide)]; poly[alpha; beta-(n-2-hydroxyethyl-dl-aspartamide)]-ketoprofen conjugate; hydrolysis; enzyme; high performance liquid chromatography
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