Effects of vanadate on glucose production in rat hepatocytes cultured in vitro (CROSBI ID 111248)
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Roša, Jagoda ; Roša, Josip
engleski
Effects of vanadate on glucose production in rat hepatocytes cultured in vitro
The aim of this study was to determine effect of vanadate on glucose production in hepatocytes cultured in vitro. Hepatocytes were isolated by a collagenase perfusion technique and cultured for 24 h in M 199 serum-free medium. The glucose production was measured by incubating the cultures in glucose-free Hanks-Hepes medium with addition 10 mmol/l pyruvate. The glucose released into the medium was determined enzymatically with glucose oxidase. Glycogenolysis was determined as net decrease in glycogen content during the incubation period. Vanadate alone in a concentration of 1 mmol/l caused a significant (p<0.01) 57% increase of glucose production during first hour, but after that period glucose production complete ceased and hepatocytes started to consume glucose, showed as a steadily and slow decrease of glucose in medium. Although insulin alone did not change glucose production, vanadate in presence of insulin even stronger (80%) increase glucose production during first hour, but after that very strongly (60%) decreased glucose in medium during second hour. Results obtained showed that vanadate decreased glucose production inhibiting gluconeogenesis
insulin; glucose production; glycogen; gluconeogenesis; glycogenolysis; vanadate
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