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Effects of troglitazone and insulin on glucose production in cultured Hepatocytes isolated from rats on high fat diet

Increased dietary fat intake in general, and saturated fat specifically, will lead to impairment of insulin action. The aim of this study was to find out changing of hepatic glucose output in dependence of fat diet and possible direct action of insulin and trogitazone in hepatocytes. Hepatocytes were isolated by a collagenase perfusion technique and cultured for 24 h in M 199 serum-free medium. The glucose production in hepatocytes isolated from rats on high fat diet (unsaturated fat) was 79% higher comparable with control and even 139% higher from rats on high fat diet (saturated fat). Troglitazone significantly decreased glucose production in hepatocytes obtained in rats on unsaturated fat diet. Troglitazone in presence of insulin totally normalized glucose production but again only in hepatocytes obtained from rats on unsaturated fat diet. Troglitazone showed an insulinomimetic as well as insulin sensitizing effect but only in rats on unsaturated fat diet.

insulin; insulin resistance; glucose production; gluconeogenesis; troglitazone

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