engleski

Tissue Expression of Proliferative Antigens (PCNA and Ki-67) in Oral Lichen Ruber to Clinical Status

Proliferative activity of epithelium cells in OLR lesions may be induced by the array of local immunological events. The aim of this study was to determine the expression intensity of PCNA and Ki- 67 tissue antigens in relation with pathologically modified oral mucosa in OLR lesions, and to determine the reaction intensity of these antigens in individual clinical forms, i.e. lichen ruber planus (LRP) and lichen ruber erosivus (LRE) comparing the reaction intensity with the inflammation grade and the degree of hyperkeratosis in lesions. The study included 30 patients with clinical and pathological diagnosis of OLR. Control group included patients (N=15) with oral leukoplakia, defined as leukoplakia simplex. Tissue antigens were observed by immunohistochemical analysis using APAAP and LSAB methods after heating in microwave oven. The reaction on tested tissue antigens was mosaic, intracellular and focal positive reaction in two or more cell populations. Antigen PCNA was discovered in basal and parabasal layer of epithelium and in inflammation infiltrate of lamina propria. The reaction was intensely high in OLR lesions regardless on the clinical form of the lesion. The reaction intensity on this tissue antigen positively correlated with the inflammation grade and the degree of hyperkeratosis in lesions. The reaction on Ki-67 tissue antigen ranged from low to moderately high intensity. Intensely high reaction was observed in lesions of lichen ruber erosivus. The reaction positively correlated with the inflammation grade and the degree of hyperkeratosis in lesions. Observed modified reaction on tissue antigens PCNA and Ki-67 related with individual clinical forms of OLR may be the indicator of transformed nature of these lesions.

oral lichen ruber planus ; proliferating cell nuclear antigens ; PCNA ; Ki-67 ; immunohistochemistry

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