engleski

Comparison of trichinelloscopy with a digestion method for the detection of Trichinella larvae in muscle tissue from naturally infected pigs with low level infections.

Control of Trichinella infection in pig has traditionally been accomplished by inspection of individual carcasses in Croatia. According to the high prevalence, all pigs slaughtered for consumption even for private purposes have to be routinely examined in order to prevent any infection in humans. In this study we tested the accuracy of trichinoscopy in samples obtained from positive pigs slaughtered for private purposes and with lower muscle larvae burden. Among 1769 muscle samples 290 were found infected with 3 or less larvae/g by a routine peptic digestion. The overall accuracy of the trichinelloscopy was 59, 48 % with the Kappa value of 0, 26 %. Sensitivity of the test was 43, 4% and specificity 88% . Artificial digestion opposite to the trichineloscopy revealed a considerable number of previously false negative animals with the burden to cause clinical trichinellosis in humans. This findings confirms previous conclusions that trichinlloscopy is not a method of choice and it is more than necessary to implement another parallel test for post slaughter control under all conditions.

Trichinelloscopy; accuracy; low larvae burden

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