engleski

Evaluation of ELISA for detection of trichinella antibodies in muscle juice samples of naturally infected pigs

The performance characteristics of an ELISA test for trichinellosis in pigs applied to muscle juice was assessed using 314 samples collected from pigs located in endemic area of Croatia. The peptic digestion assay was regarded as the reference method. The diagnostic accuracy of the two compared dilutions (1:10 and 1:100) was found high because the index AUCs was 0.922 and 0.920, respectively. In this study the two-graph receiver operating characteristic (TG-ROC analysis was used as a tool for selecting cut-off points. Sensitivity, specificity, likelihood ratios, efficiency and Youden's index were used as indices of test accuracy. The cut-off values that minimize overall misclassification cost under assumption of 3% prevalence were calculated. Our results indicate that the ELISA applied to muscle juice is a highly accurate test and can be adapted to process a large number of samples.

ELISA; muscle juice; antibodies; Trichinella

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