engleski

Comet assay in genotoxicity evaluation of alachlor in multiple mouse tissues

Pesticides of worldwide application are used in agricaulture in vast amounts each year, of which herbicides are the most prominent class. Alachlor is a chloroacetanilide, developted and introduced in 1967 by Monsanto Company from USA. It inhibits protein synthesis in young roots and is used for pre-emergence control of a broad spectrum of grass, sedge, and broadleaf weeds in corn, soybeans, dry beans, cotton, grain sorghum, sunflowers, peanuts and other crops. Due to its extensiveproduction and application of this chemical their putative detrimental effect on life should be known and minimized. In this study we applied the comet assay on blood and 4 mouse organs (kidney, liver, bone marrow, blood and spleen) to evaluate possible genome damage caused by pesticide formulations (Bravo) containing alachlor as activr compounds. Five male CBA micewere assigned to each of 4 tretment groups and control group. Bravo were injected intraperitoneally once. Bravo was given at the concentrations of 15 mg/kg and 0.01 mg/kg. Mice were sacrficed 24 hours after tretment. Alkaline comet assy on the blood samples, kidney, liver, bone marrow and spleen was performed. Statistically significant increase of tail length for all 5 tissues examined compared to the control was found. DNA of kidfney and liver showed largest increase in migration.Also, distribution of tail length values for all mouse tissues examined showed a shift to the right when compared to the controls.In conclusion, by using the alkaline comet assay on different tissues samples, genotoxic effects of pesticide in vivo can be detected and more knowledge on the specificity of its mutagenic action for different organs could be gauned. Also, used on dofferent organs in in vivo genotoxicity studies, the comet assay could provide a god assessment of potential pesticide carcinogenicity.

alachlor; CBA mice; blood; kidney; liver; bone marrow; splee; comet assayn

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