engleski

Assessment of DNA damage in leukocytes of CBA mice induced by epirubicin

The alakaline comet assay was employed to assess the primary DNA damage induced by anthracycline drug epirubicin in leukocytes of CBA mice. Inbred mouse strains have been used for many years as preferable animal models due to their cytogenetic, molecular and histopathological characteristics that are comparable to those seen in human. Epirubicin has been shown to have mutagenic and carcinogenic properties. The precise mechanism of action of epirubicin is unknown, but isprimarly releated to interacalation of the planar ring with DNA and subsequent inhibition of DNA and RNA synthesis. Genotoxic effects of epirubicin were evaluated on CBA mice with mammary carcinoma (MCa) and animals without tumor. On third and seventh day of study mice werer tretaed intravenously with epirubicin in concentration of 10 mg/kg. Corresponding negative controls were also selected. After 20 days of study blood sampling was done and alkaline comet assay was performed. To assess the levels of primary DNA damage in mice leukocytes comet tail length and tail moment were evaluated.In both epirubicin-treated groups higher levels of DNA damage compared to both negative controls were observed. Statistical analysis showed no significant differnece between DNA damage levels of both negatice controls(with or without mammary tumor).However, the levels of DNA damage recorded in epirubicin.treated mice were significantly increases compared to both negative controls. In summary, our data indicate that epirubicin induces harmful genotoxic effects in leukocytes of CBA mice. They also has s widespread use in research of genotoxic effects of differnet chemicals on animal models.

alakaline comet assay; CBA mice; epirubicin

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