engleski

Biomonitoring of genotoxic risk in radar facility workers:comparison of the comet assay with micronucleus assay and chromatid breakage assay

Genotoxic risks of occupational exposure in a radar facility were evaluated by using alkaline comet assay, micronucleus assay and chromatid breakage assay on peripheral blood leukocytes in exposed subjects and corresponding controls. Results show that occupational exposure to microwave radiation correlates with an increase of genome damage in somatic cells. The levels of DNA damage in exposed subjects determined by using alkaline comet assay were increased compared to control and showed interindividual variations. Incidence of micronuclei was also significantly increased compared to baseline control values. After short exposure of cultured Lymphocytes to bleomycin, cells of occupationally exposed subjects responded with high numbers of chromatid breaks. Although the level of chromosome damage generated by bleomycin varied greatly between individuals, in exposed subjects a significantly elevated number of chromatid breaks were observed.Our results support data reported in literature inicating that microwave radiation represents a potential DNA-damaging hazard. Alkaline comet assay is confirmed as a sensitive and gighly reproducible technique for detection of primary DNA damage inflicted in somatic cells. Micronucleus assay was confirmed as reliable biomarkers of effect and chromatid breakage assay as sensitive biomarker of individual cancer susceptibility. The results obtained also confirm the necessity measures and to perform accurate health surveillance of individuals occupationally exposed to microwave radiation.

radar; biomonitoring; comet assay; micronucleus assay; chromatid breakage assay

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