engleski

Genetic diversity of hexaploid wheat cultivars estimated by RAPD markers, morphological traits and coefficient of parentage

Estimation of genetic diversity among wheat cultivars and breeding lines and broadening their genetic basis in hexaploid wheat are essential for the future wheat breeding. Estimates of genetic diversity can be based on different types of data. The aim of this research were (1) to study genetic diversity among Croatian wheat cultivars by RAPD markers, morphological traits and pedigree records ; (2) to analyse differences between wheat cultivars from two breeding centres ; and (3) to evaluate usability of RAPD markers for estimation of genetic diversity among wheat cultivars in comparison to morphological traits and pedigree record data. Studies were conducted on 14 wheat cultivars and breeding lines from two breeding centres in Croatia. For the RAPD analysis 36 primers were screened and the 14 most polymorphic ones yielded 341 polymorphic bands. Twelve morphological traits were used for morphological analysis. Pedigrees were composed of seven generations of ancestors. RAPD markers showed a high level of polymorphism among the examined cultivars and breeding lines. No significant correlation were observed among the methods tested.

wheat ; genetic diversity ; RAPD markers ; morphological traits ; pedigree records

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