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Pregled bibliografske jedinice broj: 175399

Biochemical and functional characteristics of the photosynthetic apparatus in vegetative buds and mature needles of Norway spruce (Picea abies L. Karst.)


Lepeduš, Hrvoje; Cesar, Vera
Biochemical and functional characteristics of the photosynthetic apparatus in vegetative buds and mature needles of Norway spruce (Picea abies L. Karst.) // Acta Botanica Croatica, 63 (2004), 2; 93-99 (međunarodna recenzija, članak, znanstveni)


Naslov
Biochemical and functional characteristics of the photosynthetic apparatus in vegetative buds and mature needles of Norway spruce (Picea abies L. Karst.)

Autori
Lepeduš, Hrvoje ; Cesar, Vera

Izvornik
Acta Botanica Croatica (0365-0588) 63 (2004), 2; 93-99

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Picea abies; photosynthesis; oxigen production; respiration; NADPH-protochlorophyllide oxidoreductase; immunodetection; chlorophylls; carotenoids

Sažetak
The biochemical characteristics and functioning of the photosynthetic apparatus in two developmental stages of spruce needles (vegetative buds and mature needles) were compared. Biochemical analysis included quantification of the photosynthetic pigments and immunodetection of the NADPH-protochlorophyllide oxidoreductase (POR). Photosynthetic performance was monitored as the oxygen production at different light levels applied. Two POR isoforms were detected in buds while only one isoform was present in mature needles. The POR polypeptides in buds were expressed at different levels: the polypeptide of lower molecular mass had a level that was more enhanced than that of higher molecular mass. The most intensive POR signal was observes in mature needles. Concentrations of chlorophylls and carotenoids that were higher in mature needles than in vegetative buds were in accordance with observed POR expression. This reflected on the photosynthetic activity of the investigated organs. Although the chloroplasts in vegetative buds revealed the capability of the photosynthesising, the compensation of respiration was not observed even at the highest light level (1100 μ mol_PHOTONS m-2 s-1) applied. In contrast, mature needles compensated for respiration successfully at low light level (30 μ mol_PHOTONS m-2 s-1). Our study showed that chloroplasts in vegetative buds are photosynthetically competent, but limited by their molecular and biochemical background.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
0122016

Ustanove
Filozofski fakultet, Osijek

Profili:

Avatar Url Hrvoje Lepeduš (autor)

Avatar Url Vera Cesar (autor)

Citiraj ovu publikaciju

Lepeduš, Hrvoje; Cesar, Vera
Biochemical and functional characteristics of the photosynthetic apparatus in vegetative buds and mature needles of Norway spruce (Picea abies L. Karst.) // Acta Botanica Croatica, 63 (2004), 2; 93-99 (međunarodna recenzija, članak, znanstveni)
Lepeduš, H. & Cesar, V. (2004) Biochemical and functional characteristics of the photosynthetic apparatus in vegetative buds and mature needles of Norway spruce (Picea abies L. Karst.). Acta Botanica Croatica, 63 (2), 93-99.
@article{article, year = {2004}, pages = {93-99}, keywords = {Picea abies, photosynthesis, oxigen production, respiration, NADPH-protochlorophyllide oxidoreductase, immunodetection, chlorophylls, carotenoids}, journal = {Acta Botanica Croatica}, volume = {63}, number = {2}, issn = {0365-0588}, title = {Biochemical and functional characteristics of the photosynthetic apparatus in vegetative buds and mature needles of Norway spruce (Picea abies L. Karst.)}, keyword = {Picea abies, photosynthesis, oxigen production, respiration, NADPH-protochlorophyllide oxidoreductase, immunodetection, chlorophylls, carotenoids} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Uključenost u ostale bibliografske baze podataka:


  • Biological Abstracts