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Pregled bibliografske jedinice broj: 175008

Thin Chip Microsprayer System Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Glycopeptide Screening


Bindila, Laura; Froesch, Martin; Lion, Niels; Vukelić, Željka; Rossier, Joel; Girault, H. Hubert; Peter-Katalinić, Jasna; Zamfir, Alina
Thin Chip Microsprayer System Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Glycopeptide Screening // Rapid Communications in Mass Spectrometry, 18 (2004), 23; 2913-2920 (međunarodna recenzija, članak, znanstveni)


Naslov
Thin Chip Microsprayer System Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Glycopeptide Screening

Autori
Bindila, Laura ; Froesch, Martin ; Lion, Niels ; Vukelić, Željka ; Rossier, Joel ; Girault, H. Hubert ; Peter-Katalinić, Jasna ; Zamfir, Alina

Izvornik
Rapid Communications in Mass Spectrometry (0951-4198) 18 (2004), 23; 2913-2920

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Thin Chip Microsprayer System; FTICR Mass Spectrometry; Glycopeptide Screening

Sažetak
A thin polymer microchip was coupled with a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (FT-ICR MS) at 9.4 T and the method was optimized in negative ion mode for glycopeptide screening. The interface between the polymer microchip and FT-ICR MS consists in an in-laboratory conceived and designed mounting system that exhibits robustness and performant alignment of the chip toward the MS inlet. The particular attribute of the polymer chip coupled to FT-ICR MS to achieve an increase of ionization efficiency and sensitivity under the premise of high mass accuracy of detection is highlighted by a large number of major and minor glycopeptide structures detected and identified in highly heterogeneous mixtures obtained from urine matrices. Glycoforms expressing various saccharide chain lengths ranging from tri- to dodecasaccharide bearing up to three sialic acid moieties could be detected and according to their molecular ions assigned under the accuracy of an average mass deviation below 6 ppm. The (-)thin chipESI/FT-ICR MS is a potent novel system for glycomic screening of complex mixtures, as demonstrated for identification of single sialylated O-glycosylated amino acids and peptides from urine matrices and could be considered for general applicability in glycoanalytical field.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
0108120

Ustanove
Medicinski fakultet, Zagreb

Autor s matičnim brojem:
Željka Vukelić, (241834)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE