engleski

A thin chip microsprayer system coupled to Fourier transform ion cyclotron resonance mass spectrometry for glycopeptide screening

A thin polymer microchip was coupled with a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (FT-ICR MS) at 9.4 T and the method was optimized in negative ion mode for glycopeptide screening. The interface between the polymer microchip and FT-ICR MS consists in an in-laboratory conceived and designed mounting system that exhibits robustness and performant alignment of the chip toward the MS inlet. The particular attribute of the polymer chip coupled to FT-ICR MS to achieve an increase of ionization efficiency and sensitivity under the premise of high mass accuracy of detection is highlighted by a large number of major and minor glycopeptide structures detected and identified in highly heterogeneous mixtures obtained from urine matrices. Glycoforms expressing various saccharide chain lengths ranging from tri- to dodecasaccharide bearing up to three sialic acid moieties could be detected and according to their molecular ions assigned under the accuracy of an average mass deviation below 6 ppm. The (-)thin chipESI/FT-ICR MS is a potent novel system for glycomic screening of complex mixtures, as demonstrated for identification of single sialylated O-glycosylated amino acids and peptides from urine matrices and could be considered for general applicability in glycoanalytical field.

thin chip microsprayer system ; FTICR mass spectrometry ; glycopeptide screening

nije evidentirano