engleski

Hydrogen peroxide modulation of the superoxide anion production by stimulated neutrophils

Hydrogen peroxide (H2O2) pretreatment of human neutrophils results in a suppression of the superoxide anion (O2-) production in response to surface-acting stimulants such as lipopolysaccharide (LPS) and opsonized zymosan. This effect was not observed when phorbol myristate acetate (PMA), formyl-methionyl-leucyl-phenylalanine (fMLP) or tumor necrosis factor alfa (TNF alfa) were used as a stimuli. Since the response to PMA and other stimuli was unimpaired by preincubation with H2O2, we assume that the H2O2 modulated O2- production is probably due to alteration of the LPS receptor confirmation rather than effecting directly NADPH-oxidase. The balance of rective oxygen species (ROS) produced by neutrophils in the state of sepsis may thus be autoregulated by negative feedback phenomena of locally produced H2O2.

hydrogen peroxide; LPS; sepsis

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