engleski

Ochratoxin A in wine

A high performance liquid chromatography (HPLC) method for determination of ochratoxin A (OTA) in wine was developed and checked on 14 samples of wine produced in the Republic of Croatia.OTA detection in red and white wine was validated checking the linearity of method (r2 = 0.997 and 0.999, respectively), recovery (88 and 95 %, respectively), and precision (R.S.D. = 3.1 and 1.4 %, respectively). The detection limit for both red and white wine was 10 ng OTA/l. The OTA concentration in all red wines was above the detection limit (mean 22 &plusmn ; ; 11 ng/l), while only 4 of 7 white wines contained OTA (mean 10 &plusmn ; ; 9 ng/l). Three OTA-free white wines were from the North of Croatia (inland), while all wines produced in South Adriatic coast) were OTA-positive. However, concentrations were to low to present a considerable risk for moderate wine consumer.

analytical validation; Croatia; immunoaffinity columns; mycotoxin

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