engleski

Tumour Growth in mouse footpad - Model for radiotherapy and hyperthermia treatment

The use of hyperthermia in cancer treatment has been more studied in last two decades. Heat is frequently used with irradiation and/or cytostatcs. The aim of this study was to investigate the antitumour effect of heat, drugs and radiation on the growth of mouse mammary carcinoma and melanoma implanted into mouse footpad. When tumour volume was cca 200 mm3 in size animals were treated with heat, heat+drug and/or radiation. Drugs (cisplatin, etoposide, ifosfamide and cyclophosphamide) were dissolved in saline and given i.p. Heat was applied by immersing footpad of tumour bearing leg into water bath at 43.5°C/60 min. The end point was the time needed for treated tumour to increase its volume fivefold. The obtained data have shown that heat increases tumour growth time three times while drugs increase tumour growth time from twice to thrice. However, when heat and drugs were applied simultaneously antitumour effect was synergistic for cisplatin, ifosfamide and cyclophosphamide while etoposide gives the best antitumour effect when given 72 h before heat. If radiation and heat were combined the best antitumour effect was achieved when radiation was given 4 h before heat. When all three treatments were applied together antitumour effect was only additive.

tumour; mouse footpad; radiotherapy; hyperthermia

nije evidentirano