engleski

Cytogenetic analysis and Comet assay in detection of genome damage in medical personell occupationally exposed to antineoplastic drugs

In the present study, the method of cytogenetic analysis of peripheral blood lymphocytes and Comet assay (single cell gell electrophoresis) were used to investigate medical personell employed in application of different antineoplastic drugs and control individuals. All subjects were previously interwieved to document a history of exposure to ionizing and nonionizing radiation, chemical exposure, viral infection or special long-term medications. The chromosome aberration analysis was performed on peripheral lymphocytes cultivated in vitro for 48h. The Comet assay was performed on the same blood samples according as described by Singh et al. (1994). The exposed group showed an increased number of chromatid and chromosome breaks compared to control. The results of the Comet assay indicate that lymphocytes from exposed subjects have a greater migration of DNA (measured with the comet assay as tail length, tail moment and tail % DNA) then control lymphocytes. Results obtained show that antineoplastic drugs caused damages of DNA which could be easily detected with both methods. As a simple, rapid and sensitive method, Comet assay was more helpful in detection of genome damage and might therefore be recommended as a standard biomonitoring tool.

antineoplastic drugs; comet assay; biomonitoring

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