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The single cell gel elecrophoresis (comet assay) for detection of DNA damage in human genome


Garaj-Vrhovac, Verica; Kopjar, Nevenka; Želježić, Davor
The single cell gel elecrophoresis (comet assay) for detection of DNA damage in human genome // ..... ; knjiga sažetaka ; u: Periodicum Biologorum. Supplement, 1998. (poster, nije recenziran, sažetak, ostalo)


Naslov
The single cell gel elecrophoresis (comet assay) for detection of DNA damage in human genome

Autori
Garaj-Vrhovac, Verica ; Kopjar, Nevenka ; Želježić, Davor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
..... ; knjiga sažetaka ; u: Periodicum Biologorum. Supplement / - , 1998

Mjesto i datum
,

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Genome damage; comet assay; human lymphocyte; antineoplastic drugs

Sažetak
The Comet assay or single cell gel (SCG) assay is a new, rapid and sensitive technique for visualising and measuring DNA damage and repair in individual cells. This technique detects DNA strand breaks, alkali labile damage, incomplete excision repair sites and DNA:DNA crosslinking at the level of individual cells. Its use has increased significantly in the past few years. This technique was used to study peripheral blood cells from control subjects and subjects employed in application of antineoplastic drugs. Cells embedded in agarose on microscope slides were subjected to lysis, unwinding of DNA and electrophoresis at high pH. Comets were analysed using a fluorescence microscope and Image analysis system Comet Assay II. Our results clearly indicate that lymphocytes from exposed subjects have a greater migration of DNA (measured with the comet assay as tail length, tail moment and tail % DNA) then control lymphocytes. These preliminary results showed that antineoplastic drugs caused damages of DNA which could be easily detected with comet assay. In conclusion, the comet assay might be helpful in analysing chemical and physical characteristics of genotoxic agents and applied as a standard biomonitoring tool.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita



POVEZANOST RADA


Projekt / tema
00220107

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb