engleski

Gender differences in antioxidant capacity of rat tissues determined by 2, 2'-azinobis (3- ethylbenzothiazoline 6-sulfonate ; ABTS) and ferric reducing antioxidant power (FRAP) assays

Differences in susceptibility to oxidative stress between males and females have been postulated. Several methods have been developed to assess the total antioxidant capacity of human serum or plasma, but just recently some of them were employed for measurement of antioxidant capacity of tissues. In this study we have measured and compared antioxidant capacity of heart, kidney, liver and brain tissues of male and female rats. Antioxidant capacity has been determined using 2, 2'-azinobis (3- ethylbenzothiazoline 6-sulfonate) (ABTS) and ferric reducing antioxidant power (FRAP) assays. In the same samples, lipid peroxidation products of these tissues have been analysed using thiobarbituric acid reactive substances (TBARS) assay. Antioxidant capacity of heart, kidney and liver tissues were higher in female than male rats for both FRAP and ABTS assays. We found positive correlation between FRAP and ABTS values for all tested tissues. FRAP and ABTS proved to be comparable, simple and quick methods for antioxidant capacity scanning in tissues. TBARS levels differed only for brain tissue, being higher in males. These results indicate stronger defense against oxidative damage in females for all observed tissues. These finding may account for longer lifespan of female gender.

ABTS; Antioxidant capacity; Brain; FRAP; Gender; Heart; Kidney; Liver; TBARS

nije evidentirano