engleski

The cytogenetic characteristics of childhood lymphoma

In this report we present the results of cytomorphologic and cytogenetic investigation of non-Hodgkin lymphomas. Our investigation included 16 children. The diagnosis of malignant lymphoma was based on cytological (FNA biopsy, exfoliative or imprint method) and histopathological analysis. Cytogenetic analysis was performed on slides obtained from direct or short-term culture of lymph node and/or bone marrow cells. The results of cytological and histopathological diagnosis were compared. Correlation of the results were markedly high. Cytogenetic analysis revealed clonal chromosomal abnormalities in 68.7% of children. Chromosome instability and evidence of clonal evolution were observed in 3 patients. Pseudodiploid clone was observed in 4, hyperdiploid in 5 and hypotriploid in 2 out of 11 children with clonal chromosomal aberrations. The gains of some chromosome types were nonrandom and frequently affected chromosomes were 11, 14, 21 and X. Involvement of particular chromosome type in structural abnormalities was nonrandom too. Chromosome No.1 and No. 14 were abnormal in 5 and 4 cases respectively. This investigation confirms variability of clonal chromosomal aberrations and cytogenetic heterogeneity of childhood non-Hodgkin lymphomas. Additional investigations are however, necessary to define more precisely the frequency and types of aberrations, and biological role of chromosomal regions in the development of lymphomas.

Lymphomas; Cytogenetics

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