engleski

Sympatric Subpopulations of Botryotinia fuckeliana on Strawberries Depending on the Content of Transposable Elements and their Connection with Resistance to Botriticides

Botryotinia fuckeliana (anamorph Botrytis cinerea) is the cause of grey mould on strawberries, wich is one of the most widely occuring diseases wherever the strawberry crop is grown. The fungus manifests extraordinary genotype and phenotype variability and adaptability. One of the causes of variability is atributed to transposable elements (TE) from transposone and retrotransposone group. In connection with this fungus, two TE have been discovered so far: retrotransposons (Boty) and transposons (Flipper), based on wich the fungus Botrytis cinerea has been divided into two sympatric subpopulations: Botrytis transposa, which contains both (Boty+Flipper+), and Botrytis vacuma, which contains none (Boty-Flipper-). In order to determine to connection between these subpopulations and resistance to botryticides, we identified 184 isolates from strawberry fields that had been treated with botryticides, from groups consisting of dicarboximides, benzimidazoles, phenylsulfamides, anilinopyrimidines and hydroxianilidines. Based on this research, we determined four subpopulations of the fungus with the following content of TE: Boty+Flipper+, Boty-Flipper-, Boty+Flipper-, Boty-Flipper+. The representation of the subpopulation Botrytis transposa was 41 %, subpopulation Botrytis vacuma 23 %. The remaining two subpopulations with one of each of these elements were represented by 26 % (Boty-Flipper+) and 9 % (Boty+Flipper-). Most of the isolates proved to be resistant to at least one of the mentioned botryticides, with most of them being resistant to phenylsulfamides and benzimidazoles. The ratio of the subpopulations to botryticide resistance was different.

Sympatric subpopulations; Botrytis; Transposable elements; Strawberry

nije evidentirano