engleski

4-hydroxynonenal modifies the effects of serum growth factors on the expression of the c-fos proto-oncogene and the proliferation of HeLa cells

In this study, the effect of 4-hydroxynonenal (HNE) , a peroxidation product od 6-polyunsaturated fatty acids, on the expression of thec-fos proto-oncogene and growth factor-induced proliferation of HeL carcinoma cells in vitro was investigated. The Fos protein forms the heterodimer AP-1 with the Jun protein and regulates the cell cycle by inducing cyclin D1. Agents that are able to induce c-fos include serum, platelet-derived growth factor (PDGF), and epidermal growth factor (EGF), all od which were used in this study. The proliferation rate was determined by cell counting (viable and dead cells according to trypan blue exclusion) and the BrdU assay. The c-fos mRNA level was monitored by the reverse transcriptase/polymerase chain reaction. In the absence of HNE, serum-deprived cells responded to serum stimulation with a more than 10-fold increase of the c-fos mRNA level as well as with an increased rate of DNA synthesis and cell multiplication.

oxydative stress; c-fos; proto-oncogene; 4-hydroxynonenal; carcinoma; HeLa; cell proliferation; serum; EGF; PDGF; growtrh control; free radical

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