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Volatile metabolites associated with Aspergillus species

Aflatoxins are natural carcinogens found in the environment and are of great interest from a food safety standpoint. They are secondary metabolites produced mainly by Aspergillus flavus Link and Aspergillus parasiticus Speare. Volatile metabolites from A. flavus NRRL 3357 (aflatoxigenic) and A. flavus NRRL 1957 (non-aflatoxigenic) cultured on corn, were collected by purging and trapping headspace. Volatiles were absorbed by thermal desorption (TD) columns. Columns were then placed in a thermal desorption unit which injected the volatiles into a GC/MS for analyses and identification. In a preliminary study (after 1, 3 and 5 days) we were able to distinguish volatiles that were associated only with A. flavus (toxigenic) but not with A. flavus (nontoxigenic). After 1, 3 and 5 days, detected volatiles that were associated with toxigenic A. flavus include ethyl acetate, 3-methyl-1-butanol, 1-pentanol, 1-hexanol and nonanal, and with non-toxigenic A. flavus 2-methyl-1-propanol, 1-penten-3-ol, 2-pentanol, and 1-octene-3-ol, were identified.

Aflatoxins; Aspergillus; Volatile metabolites

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