engleski

THE EFFECT OF X-IRRADIATION ON APOPTOSIS AND PROLIFERATION OF PARENCHYMAL CELL POPULATIONS IN MOUSE SUBMANDIBULAR GLAND

Radiation-induced damage of salivary glands is seen soon after the initiation of treatment and at relatively low doses. The mechanism of this is not understood yet. Objective: The purposes of this study were to examine the apoptosis and proliferartion of parenchymal cell populations in mouse submandibular gland after X-irradiation. Methods: Submandibular glands of male C57BL/6 mice were locally X-irradiated with single dose irradiation with 7, 5 Gy. Tissue were processed for paraffin embedding at 1, 3, 6 and 10 days after X-irradiation. The apoptosis and proliferation were investigated using TUNEL method and immunohistochemistry for proliferation cell nuclear antigen (PCNA) in different gland compartments: acini, intercalated ducts and granular convoluted tubes. Results: At day 1 after irradiation, a significant decrease in PCNA-positive cells was noticed in different compartment of irradiated glands when compared to no irradiated glands (p<0, 05, for all, Post hoc test), which is indicative of the cell cycle delay. At 3 days after irradiation, the highest apoptosis in irradiated glands was noted. The number of apoptotic cells of acini, intercalated ducts and granular convoluted tubes was statistical significant higher compared to no irradiated glands (p<0, 001 for all). Furthermore, at 6 days after irradiation, the highest proliferation activity of the irradiated gland cells was noted. At 10 days after irradiation, there were no significant differences in apoptosis and proliferation between irradiated and no irradiated glands. Conclusion: Large apoptosis and proliferation in various salivary gland cell types may potentialy impair gland function.

SUBMANDIBULAR GLAND; mouse

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