engleski

Atrazine toxicity in male rat reproductive processes

Herbicide atrazine (A) effect on reproductive processes in male rat has been examined at anteroir pituitary (AP), testis (T), prostate (P) and seminal vesicles (SV). In the present study A in corn oil was administred to 90 days old rats i.p. at 120 mg/kg b.w./each 72h/60 days. Exposure of rats had induced changes in LH and FSH synthesis, changes of spermatogenesis and structural changes at all selected tissues. LH and FSH were detected by immunohistochemical method, structural changes by light and electron microscopy, while spermatogenesis has been followed by measuring concentration, total number, motility and progressive motility of sperms. A-males in AP have decreased number of immunoreactive cells for LH 25%, while FSH increased for 70%. In T control FSH is dominantly present on the perifery and is concentrated with the spermatocytes and spermatogonia. In A-rats FSH presence is within the whole irregular organized seminiferous tubules. Histochemistry of P tissue shows the tubular alveolar gland with large irregular cavities in A-rats. Our previous research on DHT-receptors in prostate showed the reduction of DHT specific binding sites on receptor molecules. At SV evidently mucosal folds are more tighty packed in A-rats vs. control. The weight of SV in A-rats was much lower and a typical atrophy of the gland is present. Inhibition of spermatogenesis in A-treated rats was in sperm count from 14.3x106 per ml vs. 17.06106 in controls. At the same time the motile sperm count has been reduced from 43% on 35%, respectively.

atrazine; rat; pituitary; testis; prostate; seminal vesicle; FSH; LH; spermatogenesis; DHT-receptors

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