engleski

Incorporation of fatty acids into tissue phospholipids in mice fed with diets rich in N-9 and N-6 fatty acids

Fats are an important component of membranes in our heart, brain, immune cells and most of the other tissues our bodies. Dietary fatty acids incorporated into membrane lipids can change membrane fluidity, which increases with the increase in desaturation of membrane lipids. The main purpose was to compare the changes in fatty acid composition of lipids induced by n-6 polyunsaturated fatty acids (PUFA, from corn oil) and n-9 monounsaturated fatty acids (MUFA, from olive oil) in polar lipids in certain tissues (liver, spleen, submandibular gland and lung). Three group of male Balb/C mice were fed for three weeks diets containing 5% (w/w) corn oil (FCO), 5% (w/w) olive oil (FOO) and control. Total lipids were extracted according to Folch et al., and polar lipids were separated and purified by solid-phase extraction and analysed in form of fatty acid methyl esters by gas chromatographic analysis.The results are shown as mean S.D. Differences among groups were tested by nonparametric Kruskal-Wallis and Mean-Whitney U-test (p  0.05). Palmitic (16:0) and stearic (18:0) fatty acids were major saturates in all polar lipids the tissue studied. Oleic acid (C18:1n-9) was the major MUFA in the tissue polar lipids varying from 7.35% (liver) up to 17.50% (submandibular gland) which was depended on enriched diets. Arachidonic acid (20:4n-6) was the major PUFA in spleen and submandibular gland tissue samples, but docosahexaenoic acid (22:6n-3) was major PUFA in lung and liver tissue samples. Like total lipids, phospholipids fatty acids affected by the type of dietary oil in each of examined tissues. The increase in C18:1n-9 content and decrease in C18:2n-6 in all examined tissues was resulted by FOO induced diet. The ration C22:6n-3/C20:4n-6 differs between tissues with the liver having the highest ratio value and spleen the lowest. The ratio were increased in the submandibular gland, by the dietary FCO, in the spleen and lung by FOO and decreased in the liver by due to the enriched oils. It is to conclude that the dietary enriched oils, type PUFA and MUFA affects fatty acid composition in the total phospholipids of liver tissue.

fatty acid; phospholipids; diet

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