engleski

Molecular analysis of the most frequent HLA-B group alleles in the Croatian population

The HLA- B4, -B7, -B8, -B12, and B35 are five of the most frequent antigens in Croatians. These alleles are very heterogenous HLA-B specificity with many subtypes which cannot be distinguished by serology and only few of them are revealed by biochemical methods. In order to define subtypes of these HLA-B antigens in Croatians, we studied 156 healthy, unrelated donors from wider area of the Croatian capital city, Zagreb. Genomic DNA was isolated by a standard salting-out method. All samples were amplified using PCR and tested with Reverse line Strip method. Among B*51 alleles, B*510l was predominant (11.9%) while B*5l02 allele occurred twice (0.6%). Allele B*0702 (9.9%) was the only observed allele between 16 different B*07 subtypes. Four different B*35 alleles were observed (B*3501-5.6%, B*3503-2.9%, B*3502-2.9%, B*3520-l.3%, while B*3508 was present once (0.3%). In the group of B8 alleles, B*080l was most frequent (2.2%), but we also observed B*0804 (l.3%) and B*0805 (0.6%). B*4402 (6.1%) occurred three times more than B*4403, and one individual had B*4407 (0.3%). Allele B*4501 was present twice (0.6%). Analysis of haplotypic associations showed that A*0201-8*5101 (2.7%), A*0301-B*0702 (3.7%), A*0201-B*3501 (2.9%), A*0101-B*080l (3.5%) and A*0201-B*4402 (1.6%) were most frequent. When HLA-A9-B12 haplotypic associations were analysed, A*2402 associated with B*4402, while B*4403 was always associated with A*2302. This molecular data will be helpful for further anthropology studies as well as for comparing findings for our population with results from other Caucasoid populations. It will also serve as a reference for donor/recipient matching in organ and bone marrow transplantation program.

HLA-B; PCR; population study; Croatians

nije evidentirano