The use of primary cell culture in testing effects of growth factor and cytotoxic effects of herbicides (CROSBI ID 500385)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Radošević, Kristina ; Gaurina Srček, Višnja ; Šimić, Branimir ; Kmetič, Ivana ; Kniewald, Jasna ; Kniewald, Zlatko
engleski
The use of primary cell culture in testing effects of growth factor and cytotoxic effects of herbicides
Proliferation of animal cells in a culture is under control of exogenous growth factors that are mostly present in a serum. The growth factors are more or less specific for particular type of cells and they are polypeptide molecules which act as physiological regulators. It is now considered that they have a substantial role in the development, tissue remodelling, tissue repair and response to injury, haematopoiesis and tumorigenesis. In our recent work, we have found out that the addition of the growth factor isolated from swine brain (BRPP = brain polypeptide), obtained by our own laboratory, has positive influence on the cell proliferation of the cell lines BHK21 C13 and CHO K1 and primary cell culture from rat's ovary. Primary cell culture can be used in the safety assessment of new chemicals, pharmaceuticals and pesticides. Today, practically in each sample of the food or water it is possible to detect very low concentrations of diferrent pesticides, in the amounts usually under the permissible levels, but even these low levels may affect the subtle biochemical processes in organism. Since in vitro methods heve been long used as a basic laboratory tools for defining biological processes, as well as of toxicological implications of xenobiotics in different cellular systems the aim of this work was to see if the growth factor isolated from swine brain (BRPP) has protective effect on cells treated with herbicide lindan. The cytotoxic effect of organochlorine herbicide lindan and protective effect of BRPP was examined on BHK21 C13 and CHO K1 cell lines and ovarian primary cell culture.
ovarian primary cell culture; herbicide citotoxicity; growth factor
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Podaci o prilogu
86-x.
2004.
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objavljeno
Podaci o matičnoj publikaciji
Abstract Book of the "3rd Croatian Congress of Toxicology - CROTOX 2004", Plitvice Lakes, Croatia, May 26-29, 2004
Zagreb: Hrvatsko toksikološko društvo
Podaci o skupu
3rd Croatian Congress of Toxicology - CROTOX 2004
poster
26.05.2004-29.05.2004
NP Plitvička jezera, Hrvatska