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Accumulation Dynamics of the Brain Total Free Fatty Acids and Free Arachidonic Acid in the Models of Cerebral Hypoxia and Epilepsy in Rats

The aim of this study was to determine the dynamics of the brain free fatty acid (FFA) pool in rats exposed to: a) controlled hypoxia and b) experimental epilepsy. Animals from group a) were subjected to hypoxia procedure. Immediately, 5, 15 or 60 minutes after loosing the righting reflex, the animals were decapitated and brains were quickly removed. Animals from group b) received penicillin (500 U/5 ul) into the left lateral ventricle and were decapitated immediately, 5 or 15 minutes after the appearance of epileptic seizures. The FFA were quantified by gas chromatography. An increase in the total brain FFA content with the maximum level at 60 minutes after losing the righting reflex was detected in hypoxia-exposed rats. The highest level of the brain free arachidonic acid (FAA) was detected 15 minutes after hypoxia. Seizures also produced an increase in the total brain FFA and brain FAA, with a statistically significant level 5 minutes after the appearance of epileptic seizures.

free fatty acids; free arachidonic acid; cerebral hypoxia; epilepsy; rats

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