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Comparative assessment of BoviPure and Percoll on bull sperm separation for fertilization in vitro

In the present study, we have examined the effect of density gradient preparations BoviPure&reg ; ; and Percoll&reg ; ; on bull sperm separation and the in vitro fertilization (IVF) results. Frozen/thawed semen from 5 Simmental bulls was pooled and sperm quality parameters were evaluated before and after separation using BoviPure&reg ; ; or Percoll&reg ; ; . We evaluated the sperm motility, the concentration, the membrane activity (HOS assay), the membrane integrity (SYBR-14/PI assay) and the acrosomal status (EthD-1/FITC-PSA assay). There were significant differences (P<0.05) between the initial and final sperm evaluation parameters after separation using BoviPure&reg ; ; and Percoll&reg ; ; , but not among them. A total of 215 oocytes were matured and fertilized in vitro and cultured in SOFaaBSA in 5 repetitions for each sperm separation method. The cleavage (D2) and blastocysts (D7) rate were significantly higher (P<0.05) for the BoviPure&reg ; ; group compared to the Percoll&reg ; ; group: 77.02% and 30.21% ; 60.18% and 20.42%, respectively. Our results indicate that using BoviPure&reg ; ; for the separation of bull sperm can improve the quality of the separated sperm and the IVF results. This finding and the similarity between those two density gradient preparations suggests that BoviPure&reg ; ; is a good alternative for sperm separation in bovine IVF.

bull; sperm; separation; Bovipure&reg;;; Percoll&reg;;; IVF

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