engleski

Effect of fumonisin B1on the micronucleus induction in rabbit kidney RK13 cell line

The mycotoxin fumonisin B1 (FB1) is known to exert adverse health effects in humans and animals. The degree of the effects depends on concentration of the toxin as well as the time of exposure. In this study rabbit kidney RK13 cells were exposed to FB1 in concentrations of 100, 200, 500 and 1000nM for 5, 24 and 48 h to evaluate its ability to induce micronuclei (MN) formation. The slides were stained with Giemsa and with DNA Fluorochrome 4, 6-diamidino-2-phenylindole (DAPI). Statistical comparisons were performed using the analysis of variance (ANOVA). The results show that FB1 caused a significant increase in MN frequency increased with dose, but not significantly. On the other hand, a clear time-dependent effect of FB1 was obtained for all doses. The frequency of MN slides stained with DAPI was significantly higher than in those stained with Giemsa, which might be due to an underestimation of small micronuclei in Giemsa-stained slides.

Fumonisin B1; micronucleus; RK13

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