engleski

Optimization of chromatographic conditions in thin-layer chromatography of flavonoids and phenolic acids

Flavonoids are one of the largest groups of natural compounds known. They are reputated to have numerous physiological activities. There are many food supplies which contain flavonoids, but one of the most important source is propolis. In propolis, main active substances except flavonoids are phenolic acids. Nowdays, the use and list of preparations of propolis became nearly endless, and demands an accurate analytical method to define the substances in this natural product. It is easily analysed by chromatographic methods, but before testing some new type of propolis it is better to optimize chromatographic conditions. The aim of this study is to optimize chromatographic conditions in TLC of flavonoids and phenolic acids as standard compounds that may be present in Croatian propolis. We used 9 different mobile phases and using information theory and numerical taxonomy methods compared by application of computer search program KT1, we found the most appropriate mobile phase, the optimal combination of two and three mobile phases for separation of standards.

flavonoids; phenolic acids; optimization; TLC; information theory; numerical taxonomy

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