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Pregled bibliografske jedinice broj: 158413

Kinetics of gas-phase hydrogen/deuterium exchange and gas-phase structure of protonated phenylalanine, proline, tyrosine and tryptophan


Rožman, Marko; Kazazić, Saša; Klasinc, Leo; Srzić, Dunja
Kinetics of gas-phase hydrogen/deuterium exchange and gas-phase structure of protonated phenylalanine, proline, tyrosine and tryptophan // RCM. Rapid communications in mass spectrometry, 17 (2003), 24; 2769-2772 doi:10.1002/rcm.1261 (međunarodna recenzija, članak, znanstveni)


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Naslov
Kinetics of gas-phase hydrogen/deuterium exchange and gas-phase structure of protonated phenylalanine, proline, tyrosine and tryptophan

Autori
Rožman, Marko ; Kazazić, Saša ; Klasinc, Leo ; Srzić, Dunja

Izvornik
RCM. Rapid communications in mass spectrometry (0951-4198) 17 (2003), 24; 2769-2772

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
hydrogen/deuterium exchange ; mass spectrometry

Sažetak
Site-specific rate constants for the gas-phase hydrogen/deuterium (H/D) exchange of four, three, five and five hydrogen atoms in protonated phenylalanine (Phe), proline (Pro), tyrosine (Tyr) and tryptophan (Trp), respectively, were determined from matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICRMS) experiments with D2O, D2S, and CH3OD as deuterating agents. No H/D exchange was observed with D2S. For exchange with both CD3OD and D2O, which is about ten times slower in the latter, results indicate for all compounds protonation of the -amino group in agreement with theoretical results. Also, with both reagents, all compounds exchange at the COOH site more than ten times faster than at the protonation site, with OH and NH sites of Tyr and Trp, respectively, exchanging slowest. The observation of H/D exchange despite the high differences in proton affinities between the amino acids and deuterating agent exceeding 200 kJ mol-1 is in agreement with lowering of the barrier for proton transfer through hydrogen bonding proposed by Lebrilla and coworkers.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekti:
0098032

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Dunja Srzić (autor)

Avatar Url Leo Klasinc (autor)

Avatar Url Marko Rožman (autor)

Avatar Url Saša Kazazić (autor)

Citiraj ovu publikaciju

Rožman, Marko; Kazazić, Saša; Klasinc, Leo; Srzić, Dunja
Kinetics of gas-phase hydrogen/deuterium exchange and gas-phase structure of protonated phenylalanine, proline, tyrosine and tryptophan // RCM. Rapid communications in mass spectrometry, 17 (2003), 24; 2769-2772 doi:10.1002/rcm.1261 (međunarodna recenzija, članak, znanstveni)
Rožman, M., Kazazić, S., Klasinc, L. & Srzić, D. (2003) Kinetics of gas-phase hydrogen/deuterium exchange and gas-phase structure of protonated phenylalanine, proline, tyrosine and tryptophan. RCM. Rapid communications in mass spectrometry, 17 (24), 2769-2772 doi:10.1002/rcm.1261.
@article{article, year = {2003}, pages = {2769-2772}, DOI = {10.1002/rcm.1261}, keywords = {hydrogen/deuterium exchange, mass spectrometry}, journal = {RCM. Rapid communications in mass spectrometry}, doi = {10.1002/rcm.1261}, volume = {17}, number = {24}, issn = {0951-4198}, title = {Kinetics of gas-phase hydrogen/deuterium exchange and gas-phase structure of protonated phenylalanine, proline, tyrosine and tryptophan}, keyword = {hydrogen/deuterium exchange, mass spectrometry} }
@article{article, year = {2003}, pages = {2769-2772}, DOI = {10.1002/rcm.1261}, keywords = {hydrogen/deuterium exchange, mass spectrometry}, journal = {RCM. Rapid communications in mass spectrometry}, doi = {10.1002/rcm.1261}, volume = {17}, number = {24}, issn = {0951-4198}, title = {Kinetics of gas-phase hydrogen/deuterium exchange and gas-phase structure of protonated phenylalanine, proline, tyrosine and tryptophan}, keyword = {hydrogen/deuterium exchange, mass spectrometry} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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