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Yeast defence mechanisms against invading exogenous DNA (CROSBI ID 498446)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija

Gjuračić, Krešimir ; Bruschi, Carlo V ; Zgaga, Zoran Yeast defence mechanisms against invading exogenous DNA // Book of abstracts of the HDBMB 2004 / Dumić, Jerka (ur.). Zagreb: Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2004. str. L11-50-x

Podaci o odgovornosti

Gjuračić, Krešimir ; Bruschi, Carlo V ; Zgaga, Zoran

engleski

Yeast defence mechanisms against invading exogenous DNA

Exchange of genetic material between different species, also called horizontal or lateral gene transfer, is considered as major force in unicellular organism evolution. In natural habitats huge variety of unicellular organisms are living together, frequently using each other as food and consequently being exposed to the exogenous DNA. One of the horizontal gene transfer mechanisms is the integration of exogenous DNA into host genome. Although this event can produce an evolutionary benefit for the host, it can also seriously compromise its genome integrity. In order to survive this threat unicellular organisms have developed numerous defence strategies against invading exogenous DNA. One of the well known strategies is the restriction-modification (R-M) system, based on discrimination between exo- and endogenous DNA by specific identification mark, and subsequent degradation of invading DNA. The R-M system is specific for bacteria with no equivalent in eukaryotic organisms. Horizontal gene transfer by means of transformation commonly occurs in contemporary molecular biology laboratories. The yeast S. cerevisiae is one of the most studied unicellular eukaryotes mainly due to relatively high efficiency of targeted genetic modifications that could be done in this organism. However, efficient yeast transformation was possible only after development of complex protocols that avoid inherent yeast defence mechanisms against invading exogenous DNA. On the basis of various yeast transformation experiments, here are proposed some initial ideas about different levels of this defence mechanisms: DNase activity associated with cell wall/periplasm compartment, ligation of incoming linear DNA with mitochondrial DNA fragments, preferential DNA integration into repetitive genomic regions and subsequent "genome cleaning" by ectopic recombination, and finally "homology search" as probably the major defence mechanism. Taken together described phenomena strongly suggest that also unicellular eukaryotes have various strategies to protect the genome integrity from exogenous DNA integration.

yeast; exogenous DNA; recombination

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Podaci o prilogu

L11-50-x.

2004.

objavljeno

Podaci o matičnoj publikaciji

Book of abstracts of the HDBMB 2004

Dumić, Jerka

Zagreb: Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu

Podaci o skupu

Congress of the Croatian Society of Biochemistry and Molecular Biology with International Participation

pozvano predavanje

03.09.2004-01.10.2004

HOC Bjelolasica, Hrvatska

Povezanost rada

Biotehnologija