engleski

Deoxyribonuclaese from Streptomyces rimosus correlates with hypothetical proteins from Streptomyces coelicolor and Streptomyces avermitilis

Background and Purpose: Streptomycetes have been shown to synthesize nonspecific endodeoxyribonucleases but there are no data on their molecular structure. The aim of this work to determine partial primary structure of the isolated DNase from Streptomyces rimosus and to search datebases for similar proteins from streptomycetes. Methods: The enzyme was isolated using standard methods for protein separation. It was cleaved by cyanogen bromide and by chymotrypsin, and amino acid sequence was determined by Edman degradation. Results: Approxymately 57 %of DNase primary structure was determined. The obtained sequence s correspond to the structure of hypothetical proteins from Streptomyces coelicolor A3 and Streptomyces avermitilis, having 62 % of identical amino acids and 71% similarity. Localization and some properties of S. rimosus DNase are similar to properties of so far described non-specific endo-DNases from Streptomyces species. Conclusion: Tentative proteins from S. coelicolor and S. avermitilis are quite certainly DNases. This finding together with data on enzymes properties, indicate the presence of a new type of endodeoxyribonucleases in streptomycetes.

Streptomyces rimosus; Deoxyribonuclease; Structure

nije evidentirano