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TLC Separation and Identification of Heavy Metals Present in Cotton Material

Optimization of separation and identification of heavy metals present on cotton material has been performed by TLC - densitometry, after microwave digestion of cotton samples. The best separation was achieved on microcrystalline cellulose pre-coated layers with acetonitrile - HCl, (conc.) - water developer and by visualization with quercetine - dimethylglioxime - NH3 reagent. A highly sensitive 3CCD color Video camera was used to capture the chromatograms under white light. Optimization of the composition of ternary mobile phase was performed using experimental design software package Design - Expert 6.

TLC; Microwave Digestion; Densitometry; Heavy metals; Cotton

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