Napredna pretraga

Pregled bibliografske jedinice broj: 15581

Ochratoxin A in blood of people from regions in Croatia with and without endemic nephropathy

Peraica, Maja; Radić, Božica; Lucić, Ana; Fuchs, Radovan; Bosanac, Ivanka; Balija, Melita; Grgičević, Damir
Ochratoxin A in blood of people from regions in Croatia with and without endemic nephropathy // Revue de Medecine Veterinaire, Vol 149. / Le Bars, Jacques ; Galtier, Pierre (ur.).
Toulouse, Francuska, 1998. (poster, međunarodna recenzija, sažetak, znanstveni)

Ochratoxin A in blood of people from regions in Croatia with and without endemic nephropathy

Peraica, Maja ; Radić, Božica ; Lucić, Ana ; Fuchs, Radovan ; Bosanac, Ivanka ; Balija, Melita ; Grgičević, Damir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Revue de Medecine Veterinaire, Vol 149. / Le Bars, Jacques ; Galtier, Pierre - Toulouse, Francuska, 1998

Mycotoxins in Food Chain, Processing and Toxicological Aspects

Mjesto i datum
Tuluz, Francuska, 02-04.07.1998.

Vrsta sudjelovanja

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Ochratoxin A; healthy population; HPLC-method

Endemic nephropathy (EN) is non-inflammatory, bilateral, interstitial, mostly tubular nephropathy occurring in rural population in the part of Republic of Croatia along the river Sava (Brodska Posavina). EN is also present in some other countries (Bulgaria, Romania, Bosnia and Herzegovina and Yugoslavia). The disease is still of unknown ethiology, although numerous authors consider that mycotoxin ochratoxin A (OTA) is involved in its ethiology. It is proved that OTA causes nephropathy in pigs and that it is nephrotoxic for all animal species investigated so far. OTA is ubiquitous mycotoxin occurring in food and feed of plant and animal origin. Humans and animals are exposed to OTA by ingestion of OTA contaminated food and feed. In humans OTA was first detected in blood of inhabitants of the endemic region in Croatia (K. HULT, R. PLEŠTINA, V. HABAZIN-NOVAK, B. RADIĆ, and S. ČEOVIĆ Arch Toxicol 51, 313-321, 1982). The presence of OA in the blood of inhabitants from the endemic and non-endemic region was checked with a spectrofluorimetric method using carboxypeptidase A, which specifically cleaves the molecule of OTA (K. HULT, S. GATENBECK J. Ass. Off. Anal. Chem. 59, 128-129, 1976). The sensitivity of this method was 2 ppb. The investigation of the presence of OTA in the inhabitants of endemic region (and control villages) was continued for twelve years. Blood was collected every year in the early spring. These investigations demonstrated that OTA is present not only in blood of people in endemic region, but also in control regions. However, the concentration of OTA in blood of persons in endemic region was higher than in control regions. In the other countries, where EN is not present (like Scandinavia, Italy, Germany etc.), OTA was found in high percentage of healthy inhabitants (A. BREITHOLTZ-EMANUELSSON: Ochratoxin A, Analysis, Occurrence and Exposure, PhD Thesis, Royal Institute of Technology, Stockholm 1994 ; J. BAUER, M. GAREIS J. Vet. Med. B 34, 613-627, 1987). In these studies much more sensitive HPLC method was used (sensitivity O.1-0.2 ng/ml), and therefore it was possible to detect very low concentrations of OTA. Recently, we have undertaken an investigation of exposure of general population in Republic of Croatia. HPLC method was used (D. BEKER and B. RADIĆ J. Chromatography 570, 441-445, 1991), the sensitivity of the method being 0.2 ng/ml. Samples of plasma were collected from healthy volunteers (blood donors) in four towns of Croatia, two belonging to the continental part of the country (Varaždin, Osijek) and two from the Mediterranean coast (Rijeka and Split). Preliminary results demonstrated that the incidence of OTA in the plasma of inhabitants from all parts of Croatia is very high, especially in the continental part of the country. However, the concentration of OTA does not exceeds 2 ng/ml., which is the sensitivity of the method used in previous investigations. Table 1. Occurrence of OTA in human plasma samples collected from plasma donors in four towns in Croatia during June 1997. City Total no. of samples Number of samples <0.2 ng/ml 0.2-1.0 ng/ml > 1.0 ng/ml Split 49 21 27 1 Rijeka 50 32 18 0 Varaždin 37 15 21 1 Osijek 50 0 41 9 Table 2. Differences in the occurrence of OTA in human plasma, calculated with Fisher's exact test (n.s.= non significant). Cities Probability Split-Rijeka-Varaždin-Osijek 5.69 x 10-13 Split-Rijeka 0.055 Split-Varaždin n.s. Split- Osijek 5.78 x 10-9 Rijeka-Varaždin 0.038 Rijeka-Osijek 2.94 x 10-14 Varazdin-Osijek 1.28 x 10-7

Izvorni jezik

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita


Projekt / tema

Institut za medicinska istraživanja i medicinu rada, Zagreb