A novel auxin conjugate hydrolase from Triticum aestivum with substrate specificity for longer side-chain auxin amide conjugates (CROSBI ID 497996)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Ludwig-Müller, Jutta ; Magnus, Volker ; Olajide, Adebanke ; Campanella, James J.
engleski
A novel auxin conjugate hydrolase from Triticum aestivum with substrate specificity for longer side-chain auxin amide conjugates
We have started to investigate how the ILR1-like indole acetic acid amidohydrolase family of genes has functionally evolved and diverged in the monocotyledonous species wheat (Triticum aestivum). We have isolated and cloned from wheat an ortholog for the Arabidopsis IAR3 auxin amidohydrolase which was called TaIAR3. TaIAR3 has no substrate specificity for any indole acetic acid (IAA) conjugates tested, unlike its ortholog IAR3. Instead, TaIAR3 has low specificity for the ester conjugates IAA-glucose and IAA-inositol and high specificity for the conjugates of indole-3-butyric acid (IBA-Alanine and IBA-Glycine) and indole-3-propionic-acid (IPA-Alanine). TaIAR3 has no substrate specificity for the methyl esters of the two IBA conjugates. IBA and IBA-Alanine were detected in wheat tissues where the conjugate may serve as a natural substrate for this enzyme. Endogenous IPA and IPA conjugates were not detected in seedlings. Additionally, crude protein extracts of wheat seedlings possess auxin amidohydrolase activity. Temporal expression studies of TaIAR3 indicate that the transcript is initially expressed at day 1 after germination. Expression down-regulates through days 2, 5, 10, 15 and 20. Spatial expression studies found similar levels of expression throughout the wheat tissues examined.
Triticum aestivum; wheat; auxin conjugate hydrolase; IAR3; indole-3-butyric acid; IBA; indole-3-propionic acid; IPA; indole-3-acetic acid; IAA; alanine; glycine
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Podaci o prilogu
41-41-x.
2004.
objavljeno
Podaci o matičnoj publikaciji
Book of Abstracts
Theologis, Athanasios ; Sandberg, Göran
Kolympari : Berkeley (CA): Agricultural Research Service, United States Department of Agriculture
Podaci o skupu
Auxin 2004
poster
22.05.2004-27.05.2004
Kolymvari, Grčka