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The growth of Aspergillus ochraceus mould cultivated in pure and mixed cultures on corn grains and the influence of certain parameters on the ochratoxin A biosynthesis


Delaš, Frane; Duraković, Senadin; Radić, Božica; Delaš, Ivančica
The growth of Aspergillus ochraceus mould cultivated in pure and mixed cultures on corn grains and the influence of certain parameters on the ochratoxin A biosynthesis // Zbornik sažetaka 3. hrvatskog kongresa prehrambenih tehnologa, biotehnologa i nutricionista / Marić, Vladimir (ur.).
Zagreb: Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, 1998. str. 125-126 (poster, nije recenziran, sažetak, znanstveni)


Naslov
The growth of Aspergillus ochraceus mould cultivated in pure and mixed cultures on corn grains and the influence of certain parameters on the ochratoxin A biosynthesis

Autori
Delaš, Frane ; Duraković, Senadin ; Radić, Božica ; Delaš, Ivančica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik sažetaka 3. hrvatskog kongresa prehrambenih tehnologa, biotehnologa i nutricionista / Marić, Vladimir - Zagreb : Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, 1998, 125-126

Skup
3. hrvatski kongres prehrambenih tehnologa, biotehnologa i nutriciniosta s međunarodnim učešćem

Mjesto i datum
Zagreb, Hrvatska, 10-12.06.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Biosynthesis; chitin; biomass; A. ochraceus. T. roseum; ochratoxin A
(Biosynthesis; chitin; biomass; A. ochraceus; T. roseum; ochratoxin A)

Sažetak
The growth of ochratoxigenic mould Aspergillus ochraceus NRRL 3174 on corn grains and in vitro contiditions for ochratoxin A (OTA) biosynthesis in pure culture as well as in the mixed culture with the nonochratoxigenic mould Trichotecium roseum ZMPBF 1226 have been investigated. The biomass growth was followed by a chitin method, while OTA concentration was measured by TLC and fluorodensitometrically. The moulds were cultivated during 35 days in a stationary culture at temperatures 15 C, 20 C, 25 C and 30 C and 38 % water content in corn grains. It was found that OTA production the temperatures between 15 C and 20 C and the water content higher than 20 % were necessary, both in the pure and the mixed culture. The highest amount of OTA produced in the pure culture was 1225 micrograms x g mycelium dry weight, and ws reached two weeks of cultivation at 20 C with 38 % water content in the corn. Under the same conditions, the maximal value obtained in the mixed culture was 790 micrograms x g mycelium dry weight reached after three weeks of incubation. The elevation of the incubation temeprature to 20 C caused 50 % increase in the biomass sytnhesis, while at the same time the OTA amount decreased 15-45 %, the most probably becaouse of the less ability of the mycelium to synthesize OTA. Athe the incubation temperature of 30 C biosynthesis was inhibited.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita



POVEZANOST RADA


Projekt / tema
00220106
058401
108182

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Prehrambeno-biotehnološki fakultet, Zagreb,
Medicinski fakultet, Zagreb