engleski

Atrazine genotoxicity evaluation in different mouse organs by comet assay

Background and purpose: Pesticides of worldwide application are used in agriculture in vast amounts each year, of which herbicides are the most prominent class. Due to extensive production and application of this chemical their putative detrimental effect on life should be known and minimized. Materials and methods: We applied the comet assay on blood and 4 mouse organs (kidney, liver, bone marrow, and spleen) to evaluate possible genome damage caused by Gesaprimâ containing atrazine as active ingredient. Male CBA mice were assigned to 4 treatment groups and control group. Gesaprimâ was injected intraperitoneally once. It was given at the dose of 1.08 ml/kg so that the dose of atrazine contained within the pesticide formulation given was 540 mg/kg and 0.07 ml of Gesaprimâ/kg so that the dose of atrazine contained within the pesticide formulation given was 3.5 &middot ; ; 10-2 mg/kg. Mice were sacrificed 24 hours after treatment. Alkaline comet assay on the blood samples, kidney, liver, bone marrow and spleen was performed. Results: Statistically significant (p < 0.01) increase of tail length for all 5 tissues examined in mice treated Gesaprimâ compared to the control was found. DNA of kidney and liver showed largest increase in migration. Also, distribution of tail length values for all mouse tissues examined showed a shift to the right when compared to the controls. Conclusions: By this work we showed that used on different organs in in vivo genotoxicity studies, the comet assay could provide a good assessment of potential pesticide carcinogenicity.

atrazin; miš; genotoksičnost; komet tehnika

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