engleski

Genotoxicity evaluation of pesticide formulations containing alachlor and atrazine in multiple mouse tissues (blood, kidney, liver, bone marrow, spleen) by comet assay

Pesticides of worldwide application are used in agriculture in vast amounts each year, of which herbicides are the most prominent class. Due to extensive production and application of this chemical their putative detrimental effect on life should be known and minimized. In this study we applied the comet assay on blood and 4 mouse organs (kidney, liver, bone marrow, and spleen) to evaluate possible genome damage caused by two pesticide formulations (Bravoâ and Gesaprimâ) containing alachlor and atrazine as active ingredients. Five male CBA mice were assigned to each of 4 treatment groups and control group. Bravoâ and Gesaprimâ were injected intraperitoneally once. Bravoâ was given at the concentrations of alachlore of 15 mg/kg and 0.01 mg/kg, whereas Gesaprimâ was given at the concentration of atrazine of 540 mg/kg and 3.5 &middot ; ; 10-2 mg/kg. Mice were sacrificed 24 hours after treatment. Alkaline comet assay on the blood samples, kidney, liver, bone marrow and spleen was performed. Statistically significant (p < 0.01) increase of tail length for all 5 tissues examined in mice treated with both Bravoâ and Gesaprimâ compared to the control was found. For both pesticides DNA of kidney and liver showed largest increase in migration. Also, distribution of tail length values for Bravoâ and Gesaprimâ for all mouse tissues examined showed a shift to the right when compared to the controls.

atrazin; alaklor; organi miša; komet; genotoksičnost

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