engleski

Induction of p53 in mussel Mytilus galloprovincialis tissues

The tumor suppressor phosphoprotein p53 as a biomarker of genotoxic stress in marine mussel Mytilus galloprovincialis were investigated. The effect of direct and indirect acting genotoxic xenobiotic compounds on p53 protein family levels were determinated using the Western blot analysis and immunoassay detection. The well known DNA damaging agents: N-methil-N´-nitro-nitrosoguanidine (MNNG), 4-nitroquinoline-N-oxide (NQO), and benzo[a]pyrene (B[a]P) induced level of p53 in a time and dose dependent manner. The aim of the study was to establish the applicability of the p53 induction as a genotoxic stress estimation tool and promised use of p53 as a biomarker in marine ecotoxicological monitoring research.

biomonitoring; ecotoxicology; genotoxic agents; musell Mytilus galloprovincialis; p53

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